People

Research Assistant

Luiza Felippi De Lima

Title

Research Assistant

Degree

Doctor in Biotechnology

Research Interests

Biosensors; Nanomaterials; Biomaterials; Ceramics; Glass-ceramics; Materials processing; Characterisation techniques; 2D materials; Graphene-based devices

Office Location

S16-06-01A

Biography

Dr Luiza Felippi de Lima is a materials scientist and biotechnologist with expertise in experimental research spanning biosensors, ceramics, composites, and the characterisation of advanced materials. She is currently a Research Assistant at the Institute for Functional Intelligent Materials (I-FIM) at the National University of Singapore (NUS), where she contributes to multidisciplinary research at the interface of materials science, biotechnology, and nanotechnology. Dr Lima obtained her PhD in Biotechnology from the University of Caxias do Sul, Brazil, where her doctoral research focused on the development of magnetoelastic biosensors for SARS-CoV-2 diagnosis. During her PhD, she was a visiting researcher at the International Iberian Nanotechnology Laboratory (INL), Portugal, working on nanomagnetic and sensor technologies. She also holds a Master’s degree in Materials Science and Engineering and a Bachelor’s degree in Materials Engineering, both from the University of Caxias do Sul. Her research interests include biosensors, nanomaterials, biomaterials, ceramics and glass-ceramics, materials processing, and advanced characterisation techniques, with a growing focus on 2D materials and graphene-based devices. Dr Lima has authored multiple publications in peer-reviewed journals and has presented her work at international conferences. Her background combines rigorous experimental skills with interdisciplinary collaboration across academia and applied research environments.

Selected Publications

Gomes Machado, K.; Lima, L. F.; de Souza, W. V.; Checca, R.; Louzada, D. R.; Sommer, R. L.; Streck, A. F.; Aguzzoli, C.; Roesch-Ely, M. Environmentally Safe Synthesis of Gold Nanoparticles for Antigen Detection in CLIA System. ACS Omega, 10 (41), 48976–48983, 2025, ISSN: 2470-1343
Ng, J. J.; Tkachev, S.; Sim, G. C.; Lima, L. F.; Koon, G. K.; Lima, A. P.; Castro Neto, A. H. Non-Invasive Hydration Monitoring with a Graphene Dual Sweat Sensor. Applied Sciences, 15 (9), 4970, 2025, ISSN: 2076-3417
Lima, L. F.; Dutra, R.; González, D.; Sommer, R. L.; Perottoni, C. A.; Aguzzoli, C.; Roesch-Ely, M. Role of extrinsic factors on magnetoelastic resonance biosensors sensitivity. Measurement Science and Technology, 35 (4), 045113, 2024, ISSN: 0957-0233
Scherer, C.; Lima, L. F.; Zorzi, J. E. Effect of partial replacement of cement by fine powders on the corrosion resistance of concrete. Construction and Building Materials, 401, 132982, 2023, ISSN: 0950-0618
Lima, L. F.; Zorzi, J. E.; Cruz, R. C. D. Basaltic glass-ceramic: A short review. Boletín de la Sociedad Española de Cerámica y Vidrio, 61, 2–12, 2022, ISSN: 0366-3175
Lima, L. F.; Perottoni, C. A.; Zorzi, J. E.; Cruz, R. C. D. Effect of iron on the microstructure of basalt glass-ceramics obtained by the petrurgic method. International Journal of Applied Ceramic Technology, 18, 1950–1959, 2021, ISSN: 1546-542X
Lima, L. F.; Mantas, P.; Segadães, A. M.; Cruz, R. C. D. Processing and characterization of sinter-crystallised basalt glass-ceramics. Journal of Non-Crystalline Solids, 538, 120019, 2020, ISSN: 0022-3093
“

I-FIM Publications:

2025

Ng, Joseph J Q; Tkachev, Sergey; Sim, Glendon C F; de Lima, Luiza Felippi; Koon, Gavin K W; Lima, Alexandre P; Neto, Antonio Castro H

Non-Invasive Hydration Monitoring with a Graphene Dual Sweat Sensor

APPLIED SCIENCES-BASEL, 15 (9), 2025, DOI: 10.3390/app15094970.

Abstract | BibTeX | Endnote

@article{WOS:001486021400001,
title = {Non-Invasive Hydration Monitoring with a Graphene Dual Sweat Sensor},
author = {Joseph J Q Ng and Sergey Tkachev and Glendon C F Sim and Luiza Felippi de Lima and Gavin K W Koon and Alexandre P Lima and Antonio Castro H Neto},
doi = {10.3390/app15094970},
times_cited = {3},
year = {2025},
date = {2025-04-01},
journal = {APPLIED SCIENCES-BASEL},
volume = {15},
number = {9},
publisher = {MDPI},
address = {MDPI AG, Grosspeteranlage 5, CH-4052 BASEL, SWITZERLAND},
abstract = {Maintaining optimal hydration is critical for physiological function,
particularly during intense physical activities, in which dehydration or
overhydration can impair performance and recovery. Traditional methods
for monitoring hydration status, such as body weight changes,
bioelectrical impedance, and urine specific gravity, are limited by
inconvenience and lack of real-time capability. This study introduces a
novel graphene-based dual-sensing electrochemical sensor for the rapid
and non-invasive quantification of sodium and potassium concentrations
in human sweat, key biomarkers of hydration status. Leveraging
graphene's exceptional conductivity and functionalization potential, the
sensor employs open-circuit potentiometry (OCP) to achieve high
sensitivity and selectivity in detecting sodium and potassium. The
sensor performance was validated against that of a commercial analyzer
and ICP-OES, demonstrating a near-Nernstian response (61.93 mV/decade
for sodium and 61.21 mV/decade for potassium detection) and a linear
detection range spanning from 0.1 mM to 100 mM for both sodium and
potassium monitoring in sweat. Sweat samples from an athlete during
endurance exercise confirmed the sensor's reliability, with results
closely matching those of ICP-OES and outperforming the commercial
analyzer in regards to accuracy and sample efficiency. This work
represents a cross-validated study of a sweat-based sensor with a second
analytical technique, highlighting its potential as a real-time
hydration monitoring tool for use in sports and beyond.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}